Biosensors and Bioelectronics

Updated: Feb 24

Biosensors and Bioelectronics 204 (2022) 114067

5.2. Quality control of CoVariant protein microarray

The CoVariant protein microarrays stored at 􀀀 80 ◦C were thawed to

room temperature, washed with 1X TBST (TBS with 0.1% Tween 20) for

10 min, blocked by SuperBlock buffer (ThermoFisher, #37537) for 15

min, and incubated with 50 μL of 647-conjugated Rabbit Anti-His tag

1.1 ng/mL (Jackson Lab, #300-605-240), anti-spike antibody 31.25 ng/

mL (Sino Biological, #40150-D001) with anti-human IgG conjugated

with Cy3 1.5 μg/mL (Jackson Laboratory, #109-165-003), or biotinylated

ACE2 125 pg/mL (Sino Biological, #10108-H08H–B) with

Cy5-conjugated Streptavidin 2 ng/mL (Jackson Lab, #016-170-084) for

quality control. After several washes, the arrays were dried and scanned

then scanned for Cy3 and Cy5 signals with power 25% and 30%

(Caduceus Biotechnology, #SpinScan).

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