Biosensors and Bioelectronics 204 (2022) 114067
5.2. Quality control of CoVariant protein microarray
The CoVariant protein microarrays stored at 80 ◦C were thawed to
room temperature, washed with 1X TBST (TBS with 0.1% Tween 20) for
10 min, blocked by SuperBlock buffer (ThermoFisher, #37537) for 15
min, and incubated with 50 μL of 647-conjugated Rabbit Anti-His tag
1.1 ng/mL (Jackson Lab, #300-605-240), anti-spike antibody 31.25 ng/
mL (Sino Biological, #40150-D001) with anti-human IgG conjugated
with Cy3 1.5 μg/mL (Jackson Laboratory, #109-165-003), or biotinylated
ACE2 125 pg/mL (Sino Biological, #10108-H08H–B) with
Cy5-conjugated Streptavidin 2 ng/mL (Jackson Lab, #016-170-084) for
quality control. After several washes, the arrays were dried and scanned
then scanned for Cy3 and Cy5 signals with power 25% and 30%
(Caduceus Biotechnology, #SpinScan).